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KMID : 0604520050310040329
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Journal of the Society of Cosmetic Scientists of Korea
2005 Volume.31 No. 4 p.329 ~ p.335
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Anti-Oxidative and Inhibitory Effect of Saussurea involucrata on MMP-1 in UVA-irradiated Human Dermal Fibroblast
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Sim Gwan-Sub
Kim Jin-Hwa
Na Young
Lee Dong-Hwan
Lee Beom-Cheon
Zhang Yong-He
Pyo Hyeong-Bae
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Abstract
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In order to investigate the effects of Saussurea involucrata on (relationship between) aging (and Saussurea involucrata), we examined the activities of antioxidation, in vitro MMP inhibition and UVA-induced MMP-1 expression in human dermal fibroblasts. S. involucrata showed scavenging activities radicals and reactive oxygen species (ROS) with the IC_{50} values of 3.89{mu}g/mL against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and 67.29{mu}g/mL against superoxide radicals in the xanthine/xanthine oxidase system, respectively. At the concentration of 1000{mu}g/mL, S. involucrata showed 93.27% inhibition on lipid peroxidation of linoleic acid. S. involucrata inhibited the activities of MMP-1 in a does-dependent manner and the IC_{50} value calculated from semi-log plots was 97.18{mu}g/mL. Also, UVA induced MMP expression in human dermal fibroblasts was reduced 42.86% by treatment with S. involucrata, and MMP-1 mRNA expression was reduced in a dose-dependent manner. Therefore S. involucrata was able to significantly inhibit MMP expression in protein and mRNA level. All these results suggested that S. involucrata might act as an anti-aging agent by antioxidation and reducing UVA-induced MMP-1 production.
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KEYWORD
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Saussurea involucrata, antioxidation, MMP, human dermal fibroblast, anti-aging
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